Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris.
Identifieur interne : 000505 ( Main/Exploration ); précédent : 000504; suivant : 000506Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris.
Auteurs : Nguyen Duc Huy [Corée du Sud] ; Seung-Wook Kim ; Seung-Moon ParkSource :
- Journal of bioscience and bioengineering [ 1347-4421 ] ; 2011.
Descripteurs français
- KwdFr :
- Clonage moléculaire (MeSH), Endo-1,4-beta xylanases (biosynthèse), Masse moléculaire (MeSH), Microbiologie industrielle (MeSH), Milieux de culture (composition chimique), Phanerochaete (enzymologie), Phanerochaete (génétique), Pichia (métabolisme), Protéines fongiques (biosynthèse), Protéines recombinantes (biosynthèse), Régions promotrices (génétique) (MeSH), Saccharomyces cerevisiae (génétique), Signaux de triage des protéines (MeSH), Vecteurs génétiques (MeSH).
- MESH :
- biosynthèse : Endo-1,4-beta xylanases, Protéines fongiques, Protéines recombinantes.
- composition chimique : Milieux de culture.
- enzymologie : Phanerochaete.
- génétique : Phanerochaete, Saccharomyces cerevisiae.
- métabolisme : Pichia.
- Clonage moléculaire, Masse moléculaire, Microbiologie industrielle, Régions promotrices (génétique), Signaux de triage des protéines, Vecteurs génétiques.
English descriptors
- KwdEn :
- Cloning, Molecular (MeSH), Culture Media (chemistry), Endo-1,4-beta Xylanases (biosynthesis), Fungal Proteins (biosynthesis), Genetic Vectors (MeSH), Industrial Microbiology (MeSH), Molecular Weight (MeSH), Phanerochaete (enzymology), Phanerochaete (genetics), Pichia (metabolism), Promoter Regions, Genetic (MeSH), Protein Sorting Signals (MeSH), Recombinant Proteins (biosynthesis), Saccharomyces cerevisiae (genetics).
- MESH :
- chemical , biosynthesis : Endo-1,4-beta Xylanases, Fungal Proteins, Recombinant Proteins.
- chemical , chemistry : Culture Media.
- enzymology : Phanerochaete.
- genetics : Phanerochaete, Saccharomyces cerevisiae.
- metabolism : Pichia.
- Cloning, Molecular, Genetic Vectors, Industrial Microbiology, Molecular Weight, Promoter Regions, Genetic, Protein Sorting Signals.
Abstract
The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500 U·l(-1).
DOI: 10.1016/j.jbiosc.2011.02.010
PubMed: 21393056
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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fongiques (biosynthèse)</term><term>Protéines recombinantes (biosynthèse)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Saccharomyces cerevisiae (génétique)</term><term>Signaux de triage des protéines (MeSH)</term><term>Vecteurs génétiques (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Endo-1,4-beta Xylanases</term><term>Fungal Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Endo-1,4-beta xylanases</term><term>Protéines fongiques</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Milieux de culture</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" 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Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500 U·l(-1).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21393056</PMID><DateCompleted><Year>2011</Year><Month>09</Month><Day>13</Day></DateCompleted><DateRevised><Year>2011</Year><Month>05</Month><Day>31</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1347-4421</ISSN><JournalIssue CitedMedium="Internet"><Volume>111</Volume><Issue>6</Issue><PubDate><Year>2011</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Journal of bioscience and bioengineering</Title><ISOAbbreviation>J Biosci Bioeng</ISOAbbreviation></Journal><ArticleTitle>Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris.</ArticleTitle><Pagination><MedlinePgn>654-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jbiosc.2011.02.010</ELocationID><Abstract><AbstractText>The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500 U·l(-1).</AbstractText><CopyrightInformation>Copyright © 2011. 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PubStatus="revised"><Year>2011</Year><Month>02</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>02</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>3</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>3</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>9</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21393056</ArticleId><ArticleId IdType="pii">S1389-1723(11)00074-0</ArticleId><ArticleId IdType="doi">10.1016/j.jbiosc.2011.02.010</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country></list><tree><noCountry><name sortKey="Kim, Seung Wook" sort="Kim, Seung Wook" uniqKey="Kim S" first="Seung-Wook" last="Kim">Seung-Wook Kim</name><name sortKey="Park, Seung Moon" sort="Park, Seung Moon" uniqKey="Park S" first="Seung-Moon" last="Park">Seung-Moon Park</name></noCountry><country name="Corée du Sud"><noRegion><name sortKey="Huy, Nguyen Duc" sort="Huy, Nguyen Duc" uniqKey="Huy N" first="Nguyen Duc" last="Huy">Nguyen Duc Huy</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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